Problems
Encountered
Following
are the some of the problems encountered in development of hybrid variety
- Farmers have to use
new hybrid seed every year. They cannot produce their own seed.
- Hybrid seed
production requires considerable technical skill. This makes hybrid
seed production a tedious and costly affair.
- The exploitation of
full potential of hybrid varieties requires an adequate supply of
irrigation water and fertilizer, and control of weeds, diseases and
insect pests. Many farmers are unable to ensure a timely application
of these essential inputs.
- The large-scale
production of hybrid seed depends on easy emasculation of the female
parent, and on an adequate pollen dispersal from the male parent. In
many species, emasculation and pollen dispersal are either
unsatisfactory or not practically feasible. In such species, e.g., in
most self-pollinated crops hybrid varieties cannot be produced on a
commercial scale unless their floral biology is adequately modified
through breeding/genetic manipulations.
- In most
cross-pollinated species, the requirements of isolation are rigid and,
ordinarily, difficult to fulfill, except on large farms. These render
hybrid seed productions rather difficult in a country like India where
farm holdings are generally small.
- The amount of
hybrid seed required to cover the entire area under any crop appears
to be impossible to produce with our present seed production setup. At
present, the total certified seed production in the country is just
enough to cover only about 4.6% of the total seed requirement of all
the crops (including self-pollinated crops, where seed production is
relatively much easier).
An
extensive use of distant hybridization in crop improvement has been
limited by several problems summarised below.
- Problems in
creating new species
Production
of new crop species through allopolyploidy following distant
hybridization suffers from several problems, e.g., lower economic
yields, poor agronomic characteristics, sterility etc. But these
characters can be improved through breeding and selection as is evident
from the history of Triticale; however, for this considerable effort,
resources and time are required.
- Dormancy
In
some cases, the F1 seeds of interspecific hybrid exhibit
dormancy. For example, some interspecific of the treatments applied for
inducing germination. Thus seed dormancy may render the use of some
interspecific F1 hybrid difficult, and in some cases, it may
effectively prevent their utilization in crop improvement.
- F1
hybrids from distant crosses generally exhibit variable sterility;
rarely the F1 is fully fertile. Partial sterility of F1
may be either selfed or backcrossed to the cultivated species. Fully
sterile F1’s are difficult to maintain, and in some cases
doubling of the chromosome number of the F1 may not be
feasible or desirable. In any case, F1 sterility confronts
the breeder with a problem to overcome which he has to resort to
specialised techniques demanding additional resources and time.
- Incompatible
Crosses
Although
many distant crosses can be made with the aid of various techniques,
several distant combinations cannot be crossed. When a cross fails, it
is generally difficult and time consuming to establish the exact cause
rally difficult and time consuming to establish the exact cause of the
failure. When the reason for the failure of a cross is not known, one
can attempt to overcome the barrier only by trial and error. Distant
hybridization is the most common, and perhaps the most useful, at the
interspecific level (within the same genus), but several intergeneric
crosses have also been of great promise, e.g., Triticale,
Raphanobrassica etc. Interfamily crosses are neither possible, and
in all probability nor profitable. A technique of somatic hybridization
is being developed to produce incompatible distant crosses, but this
technique is yet to be standardized and refined for a practical
application.
- Lack of Homology
between Chromosomes of the Parental Species
Transfer
of oligogenes from related species is mainly limited by reduced pairing
of the alien chromosome with that of the recipient species. Reduced
recombination may be overcome by chromosome manipulation, but this is
limited to polyploid species, and even there considerable cytogenetic
analysis is required to make sure that only a small segment of the alien
chromosome is transferred. In a vast majority of cases however, the gene
transfer has to depend on spontaneous pairing and recombination.
- Undesirable
linkages
Linkage
of the gene under transfer with some undesirable gene presents a major
problem in using distant hybridization in crop improvement. In fact,
undesirable linkages have prevented commercial exploitation of many
alien gene transfers. In few cases, continued breeding has been able to
break the undesirable linkages, but this demands considerable time,
effort and resources.
- Transfer of
recessive traits and of quantitative characters in many distant
hybridization programmes is not feasible. In fact, monogenic dominant
characters are the most easily transferred, and such transfers
constitute the bulk of interspecific gene transfers.
- Lack of Flowering
in F1 - F1’s from some interspecific hybrids
fail to produce flowers. Hybrids between species belonging to the
section Arachis of peanuts do not flower; all the techniques to
induce flowering in these hybrids have failed.
- In distant
hybridization programmes, it is important to use the best variety of a
crop species as one of the parents. Transfer of genes from a related
species into an agronomically poor type will necessitate further
breeding efforts to use that gene. In many cases, the best varieties
of a crop species cannot be crossed with the wild species; the wild
relatives generally cross more easily with land races (old varieties)
than with highly improved varieties.
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